Predominant transesterification reaction catalyzed by immobilized trypsin.
نویسندگان
چکیده
منابع مشابه
Transesterification of trimethylphosphate catalyzed by sodium aloxides
The transesterification process of trimethylphosphate with ethanol and isopropanol is examined. It is found that the reaction does not proceed in the absence of base even at temperature up to 100°C, whereas in the presence of sodium alkoxide as a catalyst it takes place at room temperature leading to a mixture of mono-,bi-, tri-substitute esters as well as the minor by-products resulting from t...
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The hydrolysis of a series of esters of benzoyl-L-arginine and glycylglycine by papain has been examined in mixtures of water with various alcohols in the pH range of 5 to 6. The presence of straight chain alcohols resulted in an apparent inhibition of acid release. It was shown that this inhibition could, in part, be accounted for by papain-catalyzed transesterification reactions between the v...
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متن کاملProtein hydrolysis by immobilized and stabilized trypsin.
The preparation of novel immobilized and stabilized derivatives of trypsin is reported here. The new derivatives preserved 80% of the initial catalytic activity toward synthetic substrates [benzoyl-arginine p-nitroanilide (BAPNA)] and were 50,000-fold more thermally stable than the diluted soluble enzyme in the absence of autolysis. Trypsin was immobilized on highly activated glyoxyl-Sepharose ...
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ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1984
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.32.4514